Biological activity:
Immobilized Human IL6R (1 µg/mL) bind Human IL-6 in a functional ELISA with a linear range of 2-15 ng/mL. Cell proliferation assay using TF-1 human erythroleukemic cells
Concentration:
Reconstitution dependent.
Endotoxin level:
<0.1 EU/µg of the protein by LAL method.
Expression system:
Hek293 cells
Formulation:
Lyophilized from Phosphate Buffered Saline, pH 7.4 (0.22µm filter sterilized).
Product form:
Lyophilized
Purity:
>95% (by SDS-PAGE).
Reconstitution:
Centrifuge the vial before opening and reconstitute with distilled sterile water to a concentration of 0.1-0.5 mg/mL. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer.
Sequence:
Vppgedskdvaaphrqpltsseridkqiryildgisalrketcnksnmcesskealaennlnlpkmaekdgcfqsgfneetclvkiitgllefevyleylqnrfesseeqaravqmstkvliqflqkkaknldaittpdpttnaslltklqaqnqwlqdmtthlilrsfkeflqsslralrqm
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