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The Applied Biosystems HIV-1 Genotyping Kit features:
- A standardized, cost-effective assay in a scalable workflow
- A total workflow of about 14 hours for processing 14 samples from extracted viral RNA to results, with total hands-on time of less than 3 hours
- The ability to detect mutations in the protease and reverse transcriptase regions of the HIV-1 pol gene in subtypes A, B, C, D, AE, CRF01_AE, CRF02_AG, and AG
- Easy-to-read sequencing results with free software for assembly and interpretation
Step 1 > Amplification module
After sample processing and RNA extraction, samples undergo an initial reverse transcription + PCR, followed by a second nested PCR step for mutation targets of interest. The HIV-1 Genotyping Kit Amplification Module includes the RT-PCR master mix, the nested PCR master mix, and necessary positive and negative controls that allow for a simple, streamlined workflow as you move into your cleanup and sequencing steps. Step 2 > Sequencing module
Once you confirm and clean up your targeted PCR product, the HIV-1 Genotyping Kit Sequencing Module is used for cycle sequencing. Included in this kit are the forward/reverse pre-formulated sequencing mixes for the targets of interest and the pGEM sequencing control. Step 3 > Data analysis and results
Free access to Exatype™ software is provided with the purchase of HIV-1 Genotyping Kits. This easy-to-use application allows for:
- Data upload directly to a secure web interface
- Analyzing sequencing data files, creating consensus for target regions and reporting drug resistance into a user-friendly report
- Integration with laboratory information management systems (LIMS)
- Multiple users under a single organization license
The Applied Biosystems HIV-1 Genotyping Kit features:
- A standardized, cost-effective assay in a scalable workflow
- A total workflow of about 14 hours for processing 14 samples from extracted viral RNA to results, with total hands-on time of less than 3 hours
- The ability to detect mutations in the protease and reverse transcriptase regions of the HIV-1 pol gene in subtypes A, B, C, D, AE, CRF01_AE, CRF02_AG, and AG
- Easy-to-read sequencing results with free software for assembly and interpretation
Step 1 > Amplification module
 |
After sample processing and RNA extraction, samples undergo an initial reverse transcription + PCR, followed by a second nested PCR step for mutation targets of interest. The HIV-1 Genotyping Kit Amplification Module includes the RT-PCR master mix, the nested PCR master mix, and necessary positive and negative controls that allow for a simple, streamlined workflow as you move into your cleanup and sequencing steps. |
Step 2 > Sequencing module
 |
Once you confirm and clean up your targeted PCR product, the HIV-1 Genotyping Kit Sequencing Module is used for cycle sequencing. Included in this kit are the forward/reverse pre-formulated sequencing mixes for the targets of interest and the pGEM sequencing control. |
Step 3 > Data analysis and results
|
Free access to Exatype™ software is provided with the purchase of HIV-1 Genotyping Kits. This easy-to-use application allows for:
|
The Applied Biosystems HIV-1 Genotyping Kit harnesses gold-standard Sanger sequencing technology to amplify and reliably sequence the diverse and rapidly evolving HIV-1 virus. To address the HIV surveillance needs of the global community, this kit offers broad genotyping coverage of HIV-1 Group M subtypes from plasma and dried blood spot (DBS) samples to detect resistance to protease inhibitors, nucleoside reverse-transcriptase inhibitors, and non-nucleoside reverse-transcriptase inhibitors.
This improved Applied Biosystems assay workflow was developed by Thermo Fisher Scientific in collaboration with the US Centers for Disease Control and Prevention.
Description
The Applied Biosystems HIV-1 Genotyping Kit features:
- A standardized, cost-effective assay in a scalable workflow
- A total workflow of about 14 hours for processing 14 samples from extracted viral RNA to results, with total hands-on time of less than 3 hours
- The ability to detect mutations in the protease and reverse transcriptase regions of the HIV-1 pol gene in subtypes A, B, C, D, AE, CRF01_AE, CRF02_AG, and AG
- Easy-to-read sequencing results with free software for assembly and interpretation
Step 1 > Amplification module
|
|
After sample processing and RNA extraction, samples undergo an initial reverse transcription + PCR, followed by a second nested PCR step for mutation targets of interest. The HIV-1 Genotyping Kit Amplification Module includes the RT-PCR master mix, the nested PCR master mix, and necessary positive and negative controls that allow for a simple, streamlined workflow as you move into your cleanup and sequencing steps. |
Step 2 > Sequencing module
|
|
Once you confirm and clean up your targeted PCR product, the HIV-1 Genotyping Kit Sequencing Module is used for cycle sequencing. Included in this kit are the forward/reverse pre-formulated sequencing mixes for the targets of interest and the pGEM sequencing control. |
Step 3 > Data analysis and results
|
Free access to Exatype™ software is provided with the purchase of HIV-1 Genotyping Kits. This easy-to-use application allows for:
|