- Description
Interrrogate the tumor microenvironment with an Ion Torrent short-read NGS assay
The identification of T-cell clones in tumors and the measurement of clonal expansion is important to characterize tumors, design cancer vaccines, and assess the utility for researching minimal residual disease (MRD). The CD3 region on the beta (β) chain of the T-cell receptor is most responsible for antigen recognition.
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| Oncomine TCR Beta-SR Assay | Ion GeneStudio S5 Systems |
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Characterize T cell response—measure response to tumor antigens through clonal assessment of T lymphocytes |
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Identify low frequency clones—detect T-cell malignancies with a LOD of 1 in 104 to 1 in 105 |
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Sequence the beta chain of the T-cell receptor with genomic DNA or RNA—interrogate the tumor microenvironment with 100 ng DNA or 50 ng RNA from FFPE samples |
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Oncomine TCR Beta-SR Assay DNA & RNA workflow
| Step 1 | Step 2 | Step 3 | Step 4 |
| DNA extraction OR RNA extraction | Ion AmpliSeq library creation | Ion Torrent NGS | Data analysus |
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From sample to report—in just two days
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Data security—retain full control of your data (with no service model or additional fees), full transparency in analysis, and ownership of your TCR sequences since they stay with you |
 |
Accuracy—with either DNA or RNA as a starting point, you can interrogate only the productive rearrangement of the TCR beta chain for clonality data on the Ion GeneStudio S5 System |
 |
Low input requirement—create your Ion AmpliSeq library from a range of input amounts (50 ng–1 μg), depending on your sample constraints and application preferences |
Ordering information
Description
Interrrogate the tumor microenvironment with an Ion Torrent short-read NGS assay
The identification of T-cell clones in tumors and the measurement of clonal expansion is important to characterize tumors, design cancer vaccines, and assess the utility for researching minimal residual disease (MRD). The CD3 region on the beta (β) chain of the T-cell receptor is most responsible for antigen recognition.
|
|
| Oncomine TCR Beta-SR Assay | Ion GeneStudio S5 Systems |
|
Characterize T cell response—measure response to tumor antigens through clonal assessment of T lymphocytes |
|
Identify low frequency clones—detect T-cell malignancies with a LOD of 1 in 104 to 1 in 105 |
|
Sequence the beta chain of the T-cell receptor with genomic DNA or RNA—interrogate the tumor microenvironment with 100 ng DNA or 50 ng RNA from FFPE samples |
A. |
B. |
C. |
|
|
|
Oncomine TCR Beta-SR Assay DNA & RNA workflow
| Step 1 | Step 2 | Step 3 | Step 4 |
| DNA extraction OR RNA extraction | Ion AmpliSeq library creation | Ion Torrent NGS | Data analysus |
|
|
|
|
|
|
|
|
From sample to report—in just two days
|
|
Data security—retain full control of your data (with no service model or additional fees), full transparency in analysis, and ownership of your TCR sequences since they stay with you |
|
|
Accuracy—with either DNA or RNA as a starting point, you can interrogate only the productive rearrangement of the TCR beta chain for clonality data on the Ion GeneStudio S5 System |
|
|
Low input requirement—create your Ion AmpliSeq library from a range of input amounts (50 ng–1 μg), depending on your sample constraints and application preferences |