- Description
Detection of cancer driver variants and assessment of tumor mutational burden (TMB), an emerging immuno-oncology biomarker, with next-generation sequencing
Immune checkpoint inhibitors have changed the treatment paradigm for a variety of cancers, but not all patients will respond to immunotherapies. Relevant biomarkers have shown to be as important as the therapies since they guide in our quest to help match patients with the appropriate treatment.
The Ion Torrent Oncomine Tumor Mutation Load Assay is a robust, targeted next-generation sequencing (NGS) assay designed for tumor profiling by annotation of cancer driver variants and provides accurate quantification of TMB from limited formalin- fixed, paraffin-embedded (FFPE) samples. Our streamlined, built-in analysis solution allows you to confidently detect cancer driver variants and assess tumor mutational burden in ~2.5 days for your research studies.
Video: How can the tumor mutation burden be a game changing immuno-oncology biomarker
Speaker: Dr Wolfram Jochum, Institute of Pathology, Kantonsspital St.Gallen, Switzerland
The next immuno-oncology biomarker
Prof. José Carlos Machado, PhD
Board of Directors member and Group Coordinator
Ipatimup, Porto, Portugal
Our streamlined solution allows you to confidently quantify somatic mutations and assess TMB with limited sample amounts. For maximum throughput, eight samples can be multiplexed per Ion 540 Chip, in manual and automated library preparation.
Prepare libraries with the Oncomine Tumor Mutation Load Assay, using as little as 20 ng of DNA FFPE input.
Prepare templates manually or with the Ion Chef Instrument for an automated workflow.
A carefully designed bioinformatics workflow analysis solution
Figure 1.
Assessment of tumor mutational burden: WES or targeted sequencing?
A targeted NGS panel can replace exome sequencing, bringing the advantage of a robust assay designed for oncology research specimens such as FFPE tissue. The Oncomine Tumor Mutation Load Assay enables accurate measurements of somatic mutations without the need of a matched normal sample. As panels become smaller in size, the zone of uncertainty associated with estimates of TMB expands rapidly (Figure 2A). Additionally, the coefficient of variance increases rapidly when the size of targeted panels is less than 1 Mb (Figure 2B), especially at low mutation loads. The Oncomine Tumor Mutation Load Assay covers a large genomic footprint of 1.7 Mb, encompassing 1.2 Mb of exonic sequence, to enable accurate mutation counts for samples with a range of tumor mutational burden.
Oncomine Tumor Mutation Load Assay performance: high reproducibility
High reproducbility
TMB was measured by running the assay on eight pairs of replicates. The TMB values were highly correlated (Pearson’s R = 0.99) and demonstrated reproducibility across multiple cancer types and sample types, from cell line DNA to FFPE samples (Figure 3A). The Oncomine Tumor Mutation Load Assay was tested in a series of retrospectively collected colorectal cancer samples that had been typed for microsatellite instability. As expected, high microsatellite instability samples had significantly higher TMB values than microsatellite stable samples.
| N.° de Catálogo | Nombre | Precio |
|---|---|---|
| 07237778 | ONCOMINE TML ASSAY CHEF KIT | 8.770,00 |
Description
Detection of cancer driver variants and assessment of tumor mutational burden (TMB), an emerging immuno-oncology biomarker, with next-generation sequencing
Immune checkpoint inhibitors have changed the treatment paradigm for a variety of cancers, but not all patients will respond to immunotherapies. Relevant biomarkers have shown to be as important as the therapies since they guide in our quest to help match patients with the appropriate treatment.
The Ion Torrent Oncomine Tumor Mutation Load Assay is a robust, targeted next-generation sequencing (NGS) assay designed for tumor profiling by annotation of cancer driver variants and provides accurate quantification of TMB from limited formalin- fixed, paraffin-embedded (FFPE) samples. Our streamlined, built-in analysis solution allows you to confidently detect cancer driver variants and assess tumor mutational burden in ~2.5 days for your research studies.
Video: How can the tumor mutation burden be a game changing immuno-oncology biomarker
Speaker: Dr Wolfram Jochum, Institute of Pathology, Kantonsspital St.Gallen, Switzerland
The next immuno-oncology biomarker
Prof. José Carlos Machado, PhD
Board of Directors member and Group Coordinator
Ipatimup, Porto, Portugal
Our streamlined solution allows you to confidently quantify somatic mutations and assess TMB with limited sample amounts. For maximum throughput, eight samples can be multiplexed per Ion 540 Chip, in manual and automated library preparation.
Prepare libraries with the Oncomine Tumor Mutation Load Assay, using as little as 20 ng of DNA FFPE input.
Prepare templates manually or with the Ion Chef Instrument for an automated workflow.
A carefully designed bioinformatics workflow analysis solution
Figure 1.
Assessment of tumor mutational burden: WES or targeted sequencing?
A targeted NGS panel can replace exome sequencing, bringing the advantage of a robust assay designed for oncology research specimens such as FFPE tissue. The Oncomine Tumor Mutation Load Assay enables accurate measurements of somatic mutations without the need of a matched normal sample. As panels become smaller in size, the zone of uncertainty associated with estimates of TMB expands rapidly (Figure 2A). Additionally, the coefficient of variance increases rapidly when the size of targeted panels is less than 1 Mb (Figure 2B), especially at low mutation loads. The Oncomine Tumor Mutation Load Assay covers a large genomic footprint of 1.7 Mb, encompassing 1.2 Mb of exonic sequence, to enable accurate mutation counts for samples with a range of tumor mutational burden.
Oncomine Tumor Mutation Load Assay performance: high reproducibility
High reproducbility
TMB was measured by running the assay on eight pairs of replicates. The TMB values were highly correlated (Pearson’s R = 0.99) and demonstrated reproducibility across multiple cancer types and sample types, from cell line DNA to FFPE samples (Figure 3A). The Oncomine Tumor Mutation Load Assay was tested in a series of retrospectively collected colorectal cancer samples that had been typed for microsatellite instability. As expected, high microsatellite instability samples had significantly higher TMB values than microsatellite stable samples.
| N.° de Catálogo | Nombre | Precio |
|---|---|---|
| 07237778 | ONCOMINE TML ASSAY CHEF KIT | 8.770,00 |