Oncomine™ TCR Beta-SR Assay, RNA

Oncomine™ TCR Beta-SR Assay, RNA

SKU: 07237959

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Oncomine TCR Beta-SR Assay

Interrrogate the tumor microenvironment with an Ion Torrent short-read NGS assay

The identification of T-cell clones in tumors and the measurement of clonal expansion is important to characterize tumors, design cancer vaccines, and assess the utility for researching minimal residual disease (MRD). The CD3 region on the beta (β) chain of the T-cell receptor is most responsible for antigen recognition.

The sequence coding for the CDR3 region is extremely diverse and is unique for every T-cell clonotype. Hence, in immune repertoire sequencing, the CDR3 sequences are used to identify a T-cell clonotype.  The Oncomine TCR Beta-SR Assay targets the CDR3 region, enabling such investigations.

Featured products

S5-Prime
 Oncomine TCR Beta-SR Assay Ion GeneStudio S5 Systems
immuno-oncology research Characterize T cell response—measure response to tumor antigens through clonal assessment of T lymphocytes
immunotherapy research Identify low frequency clones—detect T-cell malignancies with a LOD of 1 in 104 to 1 in 105
manufacture and function Sequence the beta chain of the T-cell receptor with genomic DNA or RNA—interrogate the tumor microenvironment with 100 ng DNA or 50 ng RNA from FFPE samples

 

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Image A Image B Image C



Oncomine TCR Beta-SR Assay DNA & RNA workflow

Step 1 Step 2 Step 3 Step 4
DNA extraction OR RNA extraction Ion AmpliSeq library creation Ion Torrent NGS Data analysus
DNA extraction Ion Ampliseq ion torrent ngs Data analysis
  • FFPE tissue
  • Fresh-frozen (FF) tissue
  • Sorted T cells
  • PBMCs/whole blood
  • cfDNA
  • For DNA preparation, libraries require 100 ng to 1 µg DNA input
  • For RNA preparation, libraries require 50 ng to 1 µg RNA input
  • Sequence samples using the Ion GeneStudio S5 System and the Ion 540 Chip or the Ion 550 Chip
  • Perform downstream data analysis with Ion Reporter Software 5.10 and up

 

From sample to report—in just two days 

data security
Data security—retain full control of your data (with no service model or additional fees), full transparency in analysis, and ownership of your TCR sequences since they stay with you
accuracy
Accuracy—with either DNA or RNA as a starting point, you can interrogate only the productive rearrangement of the TCR beta chain for clonality data on the Ion GeneStudio S5 System
low input

Low input requirement—create your Ion AmpliSeq library from a range of input amounts (50 ng–1 μg), depending on your sample constraints and application preferences

 

Ordering information

N.° de Catálogo Nombre Precio
07237959 Oncomine™ TCR Beta-SR Assay, RNA 5.120,00

Description

Oncomine TCR Beta-SR Assay

Interrrogate the tumor microenvironment with an Ion Torrent short-read NGS assay

The identification of T-cell clones in tumors and the measurement of clonal expansion is important to characterize tumors, design cancer vaccines, and assess the utility for researching minimal residual disease (MRD). The CD3 region on the beta (β) chain of the T-cell receptor is most responsible for antigen recognition.

The sequence coding for the CDR3 region is extremely diverse and is unique for every T-cell clonotype. Hence, in immune repertoire sequencing, the CDR3 sequences are used to identify a T-cell clonotype.  The Oncomine TCR Beta-SR Assay targets the CDR3 region, enabling such investigations.

Featured products

S5-Prime
 Oncomine TCR Beta-SR Assay Ion GeneStudio S5 Systems
immuno-oncology research Characterize T cell response—measure response to tumor antigens through clonal assessment of T lymphocytes
immunotherapy research Identify low frequency clones—detect T-cell malignancies with a LOD of 1 in 104 to 1 in 105
manufacture and function Sequence the beta chain of the T-cell receptor with genomic DNA or RNA—interrogate the tumor microenvironment with 100 ng DNA or 50 ng RNA from FFPE samples

 

A.
B.
C.
Image A Image B Image C



Oncomine TCR Beta-SR Assay DNA & RNA workflow

Step 1 Step 2 Step 3 Step 4
DNA extraction OR RNA extraction Ion AmpliSeq library creation Ion Torrent NGS Data analysus
DNA extraction Ion Ampliseq ion torrent ngs Data analysis
  • FFPE tissue
  • Fresh-frozen (FF) tissue
  • Sorted T cells
  • PBMCs/whole blood
  • cfDNA
  • For DNA preparation, libraries require 100 ng to 1 µg DNA input
  • For RNA preparation, libraries require 50 ng to 1 µg RNA input
  • Sequence samples using the Ion GeneStudio S5 System and the Ion 540 Chip or the Ion 550 Chip
  • Perform downstream data analysis with Ion Reporter Software 5.10 and up

 

From sample to report—in just two days 

data security
Data security—retain full control of your data (with no service model or additional fees), full transparency in analysis, and ownership of your TCR sequences since they stay with you
accuracy
Accuracy—with either DNA or RNA as a starting point, you can interrogate only the productive rearrangement of the TCR beta chain for clonality data on the Ion GeneStudio S5 System
low input

Low input requirement—create your Ion AmpliSeq library from a range of input amounts (50 ng–1 μg), depending on your sample constraints and application preferences

 

Ordering information

N.° de Catálogo Nombre Precio
07237959 Oncomine™ TCR Beta-SR Assay, RNA 5.120,00